Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1864553..1865171 Replicon chromosome
Accession NZ_CP076232
Organism Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag XE90_RS08815 Protein ID WP_001291435.1
Coordinates 1864553..1864771 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag XE90_RS08820 Protein ID WP_000344800.1
Coordinates 1864797..1865171 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XE90_RS08780 (1859840) 1859840..1860412 + 573 WP_000779831.1 YbaY family lipoprotein -
XE90_RS08785 (1860445) 1860445..1860756 - 312 WP_000409911.1 MGMT family protein -
XE90_RS08795 (1861135) 1861135..1861488 + 354 WP_000878140.1 DUF1428 domain-containing protein -
XE90_RS08800 (1861530) 1861530..1863080 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
XE90_RS08805 (1863244) 1863244..1863714 - 471 WP_000136192.1 YlaC family protein -
XE90_RS08810 (1863830) 1863830..1864381 - 552 WP_000102553.1 maltose O-acetyltransferase -
XE90_RS08815 (1864553) 1864553..1864771 - 219 WP_001291435.1 HHA domain-containing protein Toxin
XE90_RS08820 (1864797) 1864797..1865171 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
XE90_RS08825 (1865717) 1865717..1868866 - 3150 WP_226326697.1 efflux RND transporter permease AcrB -
XE90_RS08830 (1868889) 1868889..1870082 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-117)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204803 WP_001291435.1 NZ_CP076232:c1864771-1864553 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204803 NZ_CP101257:316701-317228 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204803 WP_000344800.1 NZ_CP076232:c1865171-1864797 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204803 NZ_CP101257:316428-316694 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References