Detailed information of TA system TA204801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1367915..1368465
• Replicon: chromosome
• Accession: NZ_CP076232
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: XE90_RS06485
• Protein ID: WP_001160975.1
• Coordinates: 1368151..1368465 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: XE90_RS06480
• Protein ID: WP_000125566.1
• Coordinates: 1367915..1368148 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XE90_RS06455 (1362989)	1362989..1363276	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
XE90_RS06460 (1363334)	1363334..1364665	+	1332	WP_001183177.1	MFS transporter	-
XE90_RS06465 (1364773)	1364773..1365303	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
XE90_RS06470 (1365296)	1365296..1367158	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
XE90_RS06475 (1367350)	1367350..1367829	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
XE90_RS06480 (1367915)	1367915..1368148	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
XE90_RS06485 (1368151)	1368151..1368465	+	315	WP_001160975.1	CcdB family protein	Toxin
XE90_RS06490 (1368462)	1368462..1369310	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
XE90_RS06495 (1369317)	1369317..1369694	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
XE90_RS06500 (1369697)	1369697..1370518	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
XE90_RS06505 (1370515)	1370515..1371504	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
XE90_RS06510 (1371504)	1371504..1372790	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204801 WP_001160975.1 NZ_CP076232:1368151-1368465 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204801 NZ_CP101254:c52264-51884 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204801 WP_000125566.1 NZ_CP076232:1367915-1368148 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204801 NZ_CP101254:c52485-52264 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9