Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 381487..382118 Replicon chromosome
Accession NZ_CP076232
Organism Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag XE90_RS01795 Protein ID WP_001259386.1
Coordinates 381487..381762 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag XE90_RS01800 Protein ID WP_000593555.1
Coordinates 381759..382118 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XE90_RS01780 (376491) 376491..377558 + 1068 WP_000361458.1 HlyD family secretion protein -
XE90_RS01785 (377555) 377555..380290 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
XE90_RS01790 (380290) 380290..381414 + 1125 WP_001301659.1 ABC transporter permease -
XE90_RS01795 (381487) 381487..381762 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
XE90_RS01800 (381759) 381759..382118 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
XE90_RS01805 (382168) 382168..383028 - 861 WP_001131788.1 class II aldolase -
XE90_RS01810 (383060) 383060..383329 - 270 WP_000933060.1 HPr family phosphocarrier protein -
XE90_RS01815 (383319) 383319..384827 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
XE90_RS01820 (384820) 384820..386178 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
XE90_RS01825 (386255) 386255..386536 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
XE90_RS01830 (386533) 386533..387006 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 372631..382118 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T204796 WP_001259386.1 NZ_CP076232:381487-381762 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204796 NZ_CP101251:3886286-3886438 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204796 WP_000593555.1 NZ_CP076232:381759-382118 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204796 NZ_CP101251:c3886237-3886180 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References