Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5084429..5084926 Replicon chromosome
Accession NZ_CP076230
Organism Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag XF37_RS25575 Protein ID WP_001173343.1
Coordinates 5084429..5084602 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag XF37_RS25580 Protein ID WP_000398619.1
Coordinates 5084630..5084926 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF37_RS25565 (5081099) 5081099..5082556 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
XF37_RS25570 (5082793) 5082793..5084310 + 1518 WP_001295074.1 lysine--tRNA ligase -
XF37_RS25575 (5084429) 5084429..5084602 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
XF37_RS25580 (5084630) 5084630..5084926 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
XF37_RS25585 (5085153) 5085153..5085425 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
XF37_RS25590 (5085437) 5085437..5085667 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
XF37_RS25595 (5085848) 5085848..5087479 + 1632 WP_001216477.1 sensor histidine kinase -
XF37_RS25600 (5087476) 5087476..5088195 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204793 WP_001173343.1 NZ_CP076230:c5084602-5084429 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204793 NZ_CP101251:2609452-2609559 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204793 WP_000398619.1 NZ_CP076230:c5084926-5084630 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204793 NZ_CP101251:c2609405-2609339 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References