Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 954870..955524 Replicon chromosome
Accession NZ_CP076230
Organism Escherichia coli O157:H7 strain Rafaela_II

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag XF37_RS04755 Protein ID WP_000244781.1
Coordinates 955117..955524 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag XF37_RS04750 Protein ID WP_000354046.1
Coordinates 954870..955136 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF37_RS04730 (950958) 950958..952391 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
XF37_RS04735 (952436) 952436..952747 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
XF37_RS04740 (952911) 952911..953570 + 660 WP_000250274.1 hemolysin III family protein -
XF37_RS04745 (953647) 953647..954627 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
XF37_RS04750 (954870) 954870..955136 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
XF37_RS04755 (955117) 955117..955524 + 408 WP_000244781.1 protein YgfX Toxin
XF37_RS04760 (955564) 955564..956085 - 522 WP_001055874.1 flavodoxin FldB -
XF37_RS04765 (956197) 956197..957093 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
XF37_RS04770 (957118) 957118..957828 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
XF37_RS04775 (957834) 957834..959567 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T204771 WP_000244781.1 NZ_CP076230:955117-955524 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204771 NZ_CP101245:1318196-1318594 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204771 WP_000354046.1 NZ_CP076230:954870-955136 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204771 NZ_CP101245:1317969-1318196 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References