Detailed information of TA system TA020476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2218863..2219377
• Replicon: chromosome
• Accession: NC_002758
• Organism: Staphylococcus aureus subsp. aureus Mu50

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAV_RS11415
• Protein ID: WP_000581792.1
• Coordinates: 2218863..2219072 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SAV_RS11420
• Protein ID: WP_000138932.1
• Coordinates: 2219084..2219377 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAV_RS11395	2214371..2215729	-	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAV_RS11400	2215744..2216814	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SAV_RS11405	2217132..2218334	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SAV_RS11410	2218598..2218735	-	138	WP_000828354.1	hypothetical protein	-
SAV_RS11415	2218863..2219072	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAV_RS11420	2219084..2219377	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAV_RS11425	2219543..2221027	+	1485	WP_000571549.1	cardiolipin synthase	-
SAV_RS11430	2221055..2221702	+	648	WP_001187630.1	HD domain-containing protein	-
SAV_RS11440	2222515..2223387	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAV_RS11445	2223473..2224114	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T20476 WP_000581792.1 NC_002758:c2219072-2218863 [Staphylococcus aureus subsp. aureus Mu50]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T20476 NC_002758:c2219072-2218863 [Staphylococcus aureus subsp. aureus Mu50]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT20476 WP_000138932.1 NC_002758:c2219377-2219084 [Staphylococcus aureus subsp. aureus Mu50]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT20476 NC_002758:c2219377-2219084 [Staphylococcus aureus subsp. aureus Mu50]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13