Detailed information of TA system TA204753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2315943..2316918
• Replicon: chromosome
• Accession: NZ_CP076210
• Organism: Bacillus anthracis strain A1074

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: KM397_RS12090
• Protein ID: WP_002036394.1
• Coordinates: 2315943..2316680 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: KM397_RS12095
• Protein ID: WP_000588712.1
• Coordinates: 2316793..2316918 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM397_RS12070 (KM397_12055)	2311671..2312453	+	783	WP_000381787.1	class I SAM-dependent methyltransferase	-
KM397_RS12075 (KM397_12060)	2312575..2314260	-	1686	WP_009879073.1	alpha-keto acid decarboxylase family protein	-
KM397_RS12080 (KM397_12065)	2314367..2314849	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
KM397_RS12085 (KM397_12070)	2315017..2315754	+	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
KM397_RS12090 (KM397_12075)	2315943..2316680	+	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KM397_RS12095 (KM397_12080)	2316793..2316918	+	126	WP_000588712.1	hypothetical protein	Antitoxin
KM397_RS12100 (KM397_12085)	2316941..2317171	+	231	WP_011053163.1	stage II sporulation protein SB	-
KM397_RS12105 (KM397_12090)	2317208..2317489	-	282	WP_001041555.1	hypothetical protein	-
KM397_RS12110 (KM397_12095)	2317766..2318002	-	237	WP_001021689.1	hypothetical protein	-
KM397_RS28765	2318139..2318264	+	126	WP_000694311.1	hypothetical protein	-
KM397_RS12115 (KM397_12100)	2318341..2318517	+	177	WP_000808045.1	stage II sporulation protein SB	-
KM397_RS12120 (KM397_12105)	2318661..2320130	+	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
KM397_RS12125 (KM397_12110)	2320694..2321164	-	471	WP_000670597.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T204753 WP_002036394.1 NZ_CP076210:2315943-2316680 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T204753 NZ_CP101240:1318197-1318595 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT204753 WP_000588712.1 NZ_CP076210:2316793-2316918 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT204753 NZ_CP101240:1317970-1318197 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1