Detailed information of TA system TA204751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2316778..2317753
• Replicon: chromosome
• Accession: NZ_CP076207
• Organism: Bacillus anthracis strain Pollino 3734

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: KM398_RS12115
• Protein ID: WP_002036394.1
• Coordinates: 2316778..2317515 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: KM398_RS12120
• Protein ID: WP_000588712.1
• Coordinates: 2317628..2317753 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM398_RS12095 (KM398_12090)	2312506..2313288	+	783	WP_000381787.1	class I SAM-dependent methyltransferase	-
KM398_RS12100 (KM398_12095)	2313410..2315095	-	1686	WP_014654562.1	alpha-keto acid decarboxylase family protein	-
KM398_RS12105 (KM398_12100)	2315202..2315684	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
KM398_RS12110 (KM398_12105)	2315852..2316589	+	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
KM398_RS12115 (KM398_12110)	2316778..2317515	+	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KM398_RS12120 (KM398_12115)	2317628..2317753	+	126	WP_000588712.1	hypothetical protein	Antitoxin
KM398_RS12125 (KM398_12120)	2317776..2318006	+	231	WP_011053163.1	stage II sporulation protein SB	-
KM398_RS12130 (KM398_12125)	2318043..2318324	-	282	WP_001041555.1	hypothetical protein	-
KM398_RS12135 (KM398_12130)	2318601..2318837	-	237	WP_001021689.1	hypothetical protein	-
KM398_RS28830	2318974..2319099	+	126	WP_000694311.1	hypothetical protein	-
KM398_RS12140 (KM398_12135)	2319176..2319352	+	177	WP_000808045.1	stage II sporulation protein SB	-
KM398_RS12145 (KM398_12140)	2319496..2320965	+	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
KM398_RS12150 (KM398_12145)	2321529..2321999	-	471	WP_000670597.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T204751 WP_002036394.1 NZ_CP076207:2316778-2317515 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T204751 NZ_CP101240:1020091-1020426 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT204751 WP_000588712.1 NZ_CP076207:2317628-2317753 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT204751 NZ_CP101240:1019843-1020091 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1