Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241248..241890 Replicon chromosome
Accession NZ_CP076207
Organism Bacillus anthracis strain Pollino 3734

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KM398_RS01340 Protein ID WP_000635963.1
Coordinates 241540..241890 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KM398_RS01335 Protein ID WP_000004570.1
Coordinates 241248..241535 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM398_RS01310 (KM398_01310) 236564..237526 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
KM398_RS01315 (KM398_01315) 237519..238091 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
KM398_RS01320 (KM398_01320) 238184..238543 + 360 WP_000635040.1 holo-ACP synthase -
KM398_RS01325 (KM398_01325) 238700..239650 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
KM398_RS01330 (KM398_01330) 239769..240938 + 1170 WP_000390596.1 alanine racemase -
KM398_RS01335 (KM398_01335) 241248..241535 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KM398_RS01340 (KM398_01340) 241540..241890 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KM398_RS01345 (KM398_01345) 241958..244126 + 2169 WP_000426225.1 Tex family protein -
KM398_RS01350 (KM398_01350) 244184..244300 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KM398_RS01355 (KM398_01355) 244496..244954 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T204750 WP_000635963.1 NZ_CP076207:241540-241890 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T204750 NZ_CP101240:877723-878130 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT204750 WP_000004570.1 NZ_CP076207:241248-241535 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT204750 NZ_CP101240:877476-877742 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References