Detailed information of TA system TA020475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197604..2198133
• Replicon: chromosome
• Accession: NC_002758
• Organism: Staphylococcus aureus subsp. aureus Mu50

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAV_RS11320
• Protein ID: WP_000621175.1
• Coordinates: 2197604..2197966 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAV_RS11325
• Protein ID: WP_000948331.1
• Coordinates: 2197963..2198133 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAV_RS11295	2194583..2195353	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
SAV_RS11300	2195328..2195807	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAV_RS11305	2195809..2196135	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAV_RS11310	2196254..2197255	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAV_RS11320	2197604..2197966	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAV_RS11325	2197963..2198133	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAV_RS11330	2198218..2199366	-	1149	WP_001281154.1	alanine racemase	-
SAV_RS11335	2199432..2199791	-	360	WP_000581197.1	holo-ACP synthase	-
SAV_RS11340	2199795..2200286	-	492	WP_001205912.1	PH domain-containing protein	-
SAV_RS11345	2200273..2201856	-	1584	WP_001294626.1	PH domain-containing protein	-
SAV_RS11350	2201849..2202328	-	480	WP_001287087.1	hypothetical protein	-
SAV_RS11355	2202536..2203096	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T20475 WP_000621175.1 NC_002758:c2197966-2197604 [Staphylococcus aureus subsp. aureus Mu50]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T20475 NC_002758:c2197966-2197604 [Staphylococcus aureus subsp. aureus Mu50]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT20475 WP_000948331.1 NC_002758:c2198133-2197963 [Staphylococcus aureus subsp. aureus Mu50]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT20475 NC_002758:c2198133-2197963 [Staphylococcus aureus subsp. aureus Mu50]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1