Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241250..241892 Replicon chromosome
Accession NZ_CP076178
Organism Bacillus anthracis strain BUL 12

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KM402_RS01340 Protein ID WP_000635963.1
Coordinates 241542..241892 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KM402_RS01335 Protein ID WP_000004570.1
Coordinates 241250..241537 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM402_RS01310 (KM402_01310) 236566..237528 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
KM402_RS01315 (KM402_01315) 237521..238093 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
KM402_RS01320 (KM402_01320) 238186..238545 + 360 WP_000635040.1 holo-ACP synthase -
KM402_RS01325 (KM402_01325) 238702..239652 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
KM402_RS01330 (KM402_01330) 239771..240940 + 1170 WP_000390596.1 alanine racemase -
KM402_RS01335 (KM402_01335) 241250..241537 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KM402_RS01340 (KM402_01340) 241542..241892 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KM402_RS01345 (KM402_01345) 241960..244128 + 2169 WP_000426225.1 Tex family protein -
KM402_RS01350 (KM402_01350) 244186..244302 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KM402_RS01355 (KM402_01355) 244498..244956 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T204730 WP_000635963.1 NZ_CP076178:241542-241892 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T204730 NZ_CP101233:667387-667701 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT204730 WP_000004570.1 NZ_CP076178:241250-241537 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT204730 NZ_CP101233:667698-668114 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References