Detailed information of TA system TA204727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2311510..2312485
• Replicon: chromosome
• Accession: NZ_CP076173
• Organism: Bacillus anthracis strain A178

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: KM417_RS12075
• Protein ID: WP_002036394.1
• Coordinates: 2311510..2312247 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: KM417_RS12080
• Protein ID: WP_000588712.1
• Coordinates: 2312360..2312485 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM417_RS12055 (KM417_12060)	2307238..2308020	+	783	WP_000381787.1	class I SAM-dependent methyltransferase	-
KM417_RS12060 (KM417_12065)	2308142..2309827	-	1686	WP_014654562.1	alpha-keto acid decarboxylase family protein	-
KM417_RS12065 (KM417_12070)	2309934..2310416	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
KM417_RS12070 (KM417_12075)	2310584..2311321	+	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
KM417_RS12075 (KM417_12080)	2311510..2312247	+	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KM417_RS12080 (KM417_12085)	2312360..2312485	+	126	WP_000588712.1	hypothetical protein	Antitoxin
KM417_RS12085 (KM417_12090)	2312508..2312738	+	231	WP_011053163.1	stage II sporulation protein SB	-
KM417_RS12090 (KM417_12095)	2312775..2313056	-	282	WP_001041555.1	hypothetical protein	-
KM417_RS12095 (KM417_12100)	2313333..2313569	-	237	WP_001021689.1	hypothetical protein	-
KM417_RS28735	2313706..2313831	+	126	WP_000694311.1	hypothetical protein	-
KM417_RS12100 (KM417_12105)	2313908..2314084	+	177	WP_000808045.1	stage II sporulation protein SB	-
KM417_RS12105 (KM417_12110)	2314228..2315697	+	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
KM417_RS12110 (KM417_12115)	2316261..2316731	-	471	WP_000670597.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T204727 WP_002036394.1 NZ_CP076173:2311510-2312247 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T204727 NZ_CP101227:c29789-29529 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATTTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGAAAAAAATGCTAACTGA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT204727 WP_000588712.1 NZ_CP076173:2312360-2312485 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT204727 NZ_CP101227:c29539-29288 [Escherichia coli]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1