Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241223..241865 Replicon chromosome
Accession NZ_CP076144
Organism Bacillus anthracis strain 3016

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KM411_RS01340 Protein ID WP_000635963.1
Coordinates 241515..241865 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KM411_RS01335 Protein ID WP_000004570.1
Coordinates 241223..241510 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM411_RS01310 (KM411_01310) 236539..237501 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
KM411_RS01315 (KM411_01315) 237494..238066 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
KM411_RS01320 (KM411_01320) 238159..238518 + 360 WP_000635040.1 holo-ACP synthase -
KM411_RS01325 (KM411_01325) 238675..239625 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
KM411_RS01330 (KM411_01330) 239744..240913 + 1170 WP_000390596.1 alanine racemase -
KM411_RS01335 (KM411_01335) 241223..241510 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KM411_RS01340 (KM411_01340) 241515..241865 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KM411_RS01345 (KM411_01345) 241933..244101 + 2169 WP_000426225.1 Tex family protein -
KM411_RS01350 (KM411_01350) 244159..244275 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KM411_RS01355 (KM411_01355) 244471..244929 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T204704 WP_000635963.1 NZ_CP076144:241515-241865 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T204704 NZ_CP101222:c1596561-1596175 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT204704 WP_000004570.1 NZ_CP076144:241223-241510 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT204704 NZ_CP101222:c1596185-1595856 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References