Detailed information of TA system TA204699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4845801..4846363
• Replicon: chromosome
• Accession: NZ_CP076136
• Organism: Bradyrhizobium sediminis strain S2-11-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KMZ93_RS23335
• Protein ID: WP_215603615.1
• Coordinates: 4845801..4846172 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KMZ93_RS23340
• Protein ID: WP_215603616.1
• Coordinates: 4846172..4846363 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ93_RS23305 (KMZ93_23305)	4841970..4842662	+	693	WP_215603609.1	hypothetical protein	-
KMZ93_RS23310 (KMZ93_23310)	4842788..4843450	-	663	WP_215603610.1	TetR/AcrR family transcriptional regulator	-
KMZ93_RS23315 (KMZ93_23315)	4843547..4844191	+	645	WP_215603611.1	glutathione binding-like protein	-
KMZ93_RS23320 (KMZ93_23320)	4844230..4844796	-	567	WP_215603612.1	TetR/AcrR family transcriptional regulator	-
KMZ93_RS23325 (KMZ93_23325)	4844805..4845272	-	468	WP_215603613.1	nuclear transport factor 2 family protein	-
KMZ93_RS23330 (KMZ93_23330)	4845346..4845795	-	450	WP_215603614.1	tol-pal system-associated acyl-CoA thioesterase	-
KMZ93_RS23335 (KMZ93_23335)	4845801..4846172	-	372	WP_215603615.1	PIN domain-containing protein	Toxin
KMZ93_RS23340 (KMZ93_23340)	4846172..4846363	-	192	WP_215603616.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KMZ93_RS23345 (KMZ93_23345)	4846448..4847365	-	918	WP_215603617.1	metallophosphoesterase	-
KMZ93_RS23350 (KMZ93_23350)	4847462..4848511	-	1050	WP_215603618.1	Holliday junction branch migration DNA helicase RuvB	-
KMZ93_RS23355 (KMZ93_23355)	4848508..4849125	-	618	WP_215603619.1	Holliday junction branch migration protein RuvA	-
KMZ93_RS23360 (KMZ93_23360)	4849122..4849649	-	528	WP_215603620.1	crossover junction endodeoxyribonuclease RuvC	-
KMZ93_RS23365 (KMZ93_23365)	4849829..4850575	-	747	WP_215603621.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13544.68 Da
• Isoelectric Point: 4.7078

>T204699 WP_215603615.1 NZ_CP076136:c4846172-4845801 [Bradyrhizobium sediminis]
MVLADTSIWIDHFRRGDFQLSELLERGDIVMHPFIVGELLLGHVPRIAEMLDDLNVLPRAIVASAEEVLKFISDRKLPGS
GIGYVDAHLLAAAALTPETSVWTRDKRLLAAAQALSLATEFPE

Nucleotide

• Length: 372 bp

>T204699 NZ_CP101222:455647-455997 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7089.22 Da
• Isoelectric Point: 11.1982

>AT204699 WP_215603616.1 NZ_CP076136:c4846363-4846172 [Bradyrhizobium sediminis]
MRTTIAIDDELFAKAQEFAGVTEKSAVIREALKAFVEREAARRLARMGGTQPEAKAPPRRRFK

Nucleotide

• Length: 192 bp

>AT204699 NZ_CP101222:455402-455653 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA