Detailed information of TA system TA204690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 3431836..3432428
• Replicon: chromosome
• Accession: NZ_CP076134
• Organism: Bradyrhizobium sediminis strain S2-20-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KMZ29_RS16650
• Protein ID: WP_215615945.1
• Coordinates: 3432240..3432428 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KMZ29_RS16645
• Protein ID: WP_215620250.1
• Coordinates: 3431836..3432234 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ29_RS26885	3426947..3427069	+	123	WP_256442489.1	hypothetical protein	-
KMZ29_RS16630 (KMZ29_16630)	3427319..3428821	+	1503	WP_215620247.1	replicative DNA helicase	-
KMZ29_RS16635 (KMZ29_16635)	3428870..3430117	+	1248	WP_215620248.1	alanine racemase	-
KMZ29_RS16640 (KMZ29_16640)	3430259..3431548	+	1290	WP_215620249.1	hemolysin family protein	-
KMZ29_RS16645 (KMZ29_16645)	3431836..3432234	-	399	WP_215620250.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KMZ29_RS16650 (KMZ29_16650)	3432240..3432428	-	189	WP_215615945.1	type II toxin-antitoxin system HicA family toxin	Toxin
KMZ29_RS16655 (KMZ29_16655)	3432670..3434145	+	1476	WP_215615944.1	DNA repair protein RadA	-
KMZ29_RS16660 (KMZ29_16660)	3434306..3434935	+	630	WP_215620251.1	CvpA family protein	-
KMZ29_RS16665 (KMZ29_16665)	3434968..3436476	+	1509	WP_215624308.1	amidophosphoribosyltransferase	-
KMZ29_RS16670 (KMZ29_16670)	3436619..3437359	+	741	WP_215620252.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7135.32 Da
• Isoelectric Point: 11.6831

>T204690 WP_215615945.1 NZ_CP076134:c3432428-3432240 [Bradyrhizobium sediminis]
MKSREIISALQRDGWHEVARKGSHVQFKHPRKKGRVTVPHPERDIPVGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T204690 NZ_CP101210:c4563557-4563384 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 13876.61 Da
• Isoelectric Point: 4.1863

>AT204690 WP_215620250.1 NZ_CP076134:c3432234-3431836 [Bradyrhizobium sediminis]
MGQYIGLIHKEADSHFGVSFPDFPGVATAGTTLDDARAMAEEALAFHIEGLVEDGEAIPEPSSLEDVMADPDNQSGVAIL
VAAKTEAPKVVRVNVTLPGDILEQIDKYAEAHGLSRSGFLTQAAKKAMLEAA

Nucleotide

• Length: 399 bp

>AT204690 NZ_CP101210:c4563881-4563585 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG