Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7023..7548 Replicon plasmid pF11A
Accession NZ_CP076124
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag F11EC_RS24150 Protein ID WP_001159871.1
Coordinates 7023..7328 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag F11EC_RS24155 Protein ID WP_000813634.1
Coordinates 7330..7548 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS24120 (2290) 2290..3560 + 1271 Protein_4 translesion error-prone DNA polymerase V subunit UmuC -
F11EC_RS24125 (3565) 3565..3957 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
F11EC_RS24130 (3962) 3962..4933 - 972 WP_001103694.1 plasmid segregation protein ParM -
F11EC_RS24135 (5162) 5162..5806 + 645 WP_000633911.1 AAA family ATPase -
F11EC_RS24140 (5800) 5800..6075 + 276 WP_000239529.1 hypothetical protein -
F11EC_RS24145 (6213) 6213..7022 - 810 WP_000016979.1 site-specific integrase -
F11EC_RS24150 (7023) 7023..7328 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
F11EC_RS24155 (7330) 7330..7548 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F11EC_RS24160 (9870) 9870..10511 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114223 114223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T204685 WP_001159871.1 NZ_CP076124:c7328-7023 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T204685 NZ_CP101210:3662165-3662383 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT204685 WP_000813634.1 NZ_CP076124:c7548-7330 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT204685 NZ_CP101210:3661765-3662139 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCACGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References