Detailed information of TA system TA204674

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3279890..3280725
• Replicon: chromosome
• Accession: NZ_CP076123
• Organism: Escherichia coli strain F11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: F11EC_RS15665
• Protein ID: WP_001094445.1
• Coordinates: 3280348..3280725 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: F11EC_RS15660
• Protein ID: WP_024174331.1
• Coordinates: 3279890..3280258 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11EC_RS15635 (3277315)	3277315..3277548	+	234	WP_001117568.1	DUF905 family protein	-
F11EC_RS15640 (3277639)	3277639..3278457	+	819	WP_001234749.1	DUF932 domain-containing protein	-
F11EC_RS15645 (3278549)	3278549..3279034	+	486	WP_000214415.1	antirestriction protein	-
F11EC_RS15650 (3279050)	3279050..3279526	+	477	WP_001186725.1	RadC family protein	-
F11EC_RS15655 (3279595)	3279595..3279816	+	222	WP_000692346.1	DUF987 domain-containing protein	-
F11EC_RS15660 (3279890)	3279890..3280258	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F11EC_RS15665 (3280348)	3280348..3280725	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
F11EC_RS15670 (3280722)	3280722..3281210	+	489	WP_000761718.1	DUF5983 family protein	-
F11EC_RS15675 (3281222)	3281222..3281419	+	198	WP_000838536.1	DUF957 domain-containing protein	-
F11EC_RS15680 (3281504)	3281504..3282346	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
F11EC_RS15685 (3282689)	3282689..3282859	+	171	Protein_3089	IS110 family transposase	-
F11EC_RS15690 (3283655)	3283655..3284638	+	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3282755..3282859	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T204674 WP_001094445.1 NZ_CP076123:3280348-3280725 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T204674 NZ_CP101210:1017326-1017733 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT204674 WP_024174331.1 NZ_CP076123:3279890-3280258 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT204674 NZ_CP101210:1017079-1017345 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6