Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3166144..3166798 Replicon chromosome
Accession NZ_CP076123
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag F11EC_RS15045 Protein ID WP_000244765.1
Coordinates 3166144..3166551 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F11EC_RS15050 Protein ID WP_000354046.1
Coordinates 3166532..3166798 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS15025 (3162101) 3162101..3163834 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
F11EC_RS15030 (3163840) 3163840..3164550 - 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F11EC_RS15035 (3164575) 3164575..3165471 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
F11EC_RS15040 (3165583) 3165583..3166104 + 522 WP_001055874.1 flavodoxin FldB -
F11EC_RS15045 (3166144) 3166144..3166551 - 408 WP_000244765.1 protein YgfX Toxin
F11EC_RS15050 (3166532) 3166532..3166798 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F11EC_RS15055 (3167041) 3167041..3168021 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
F11EC_RS15060 (3168098) 3168098..3168757 - 660 WP_000250274.1 hemolysin III family protein -
F11EC_RS15065 (3168921) 3168921..3169232 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
F11EC_RS15070 (3169277) 3169277..3170710 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T204673 WP_000244765.1 NZ_CP076123:c3166551-3166144 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204673 NZ_CP101210:c863599-863222 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204673 WP_000354046.1 NZ_CP076123:c3166798-3166532 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204673 NZ_CP101210:c864020-863646 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References