Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1644968..1646556 Replicon chromosome
Accession NZ_CP076123
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag F11EC_RS07720 Protein ID WP_001125432.1
Coordinates 1645234..1646556 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag F11EC_RS07715 Protein ID WP_001304368.1
Coordinates 1644968..1645234 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS07700 (1641336) 1641336..1642787 + 1452 WP_000854642.1 tagaturonate reductase -
F11EC_RS07705 (1642984) 1642984..1643898 + 915 WP_001298637.1 bestrophin family protein -
F11EC_RS07710 (1643902) 1643902..1644660 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
F11EC_RS07715 (1644968) 1644968..1645234 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
F11EC_RS07720 (1645234) 1645234..1646556 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
F11EC_RS07725 (1646732) 1646732..1647424 - 693 WP_000382466.1 hypothetical protein -
F11EC_RS07730 (1647436) 1647436..1647711 - 276 WP_000948310.1 hypothetical protein -
F11EC_RS07735 (1647713) 1647713..1647853 - 141 WP_001335097.1 hypothetical protein -
F11EC_RS07740 (1647969) 1647969..1648151 - 183 WP_001335098.1 hypothetical protein -
F11EC_RS07745 (1648321) 1648321..1649373 + 1053 WP_000568119.1 phage/plasmid replication protein, II/X family -
F11EC_RS07750 (1649385) 1649385..1649702 + 318 WP_000073834.1 DNA-binding protein -
F11EC_RS07755 (1649906) 1649906..1650148 + 243 WP_000699011.1 phage capsid protein -
F11EC_RS07760 (1650195) 1650195..1651474 + 1280 Protein_1523 attachment protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1644950..1683232 38282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T204667 WP_001125432.1 NZ_CP076123:1645234-1646556 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T204667 NZ_CP101210:272384-272491 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT204667 WP_001304368.1 NZ_CP076123:1644968-1645234 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT204667 NZ_CP101210:c272336-272270 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References