Detailed information of TA system TA204658

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1073618..1074449
• Replicon: chromosome
• Accession: NZ_CP076123
• Organism: Escherichia coli strain F11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: F11EC_RS04995
• Protein ID: WP_000854814.1
• Coordinates: 1073618..1073992 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: F11EC_RS05000
• Protein ID: WP_024174331.1
• Coordinates: 1074081..1074449 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11EC_RS04955 (1069014)	1069014..1070180	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
F11EC_RS04960 (1070299)	1070299..1070772	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
F11EC_RS04965 (1070970)	1070970..1072028	+	1059	WP_001200890.1	FUSC family protein	-
F11EC_RS04970 (1072200)	1072200..1072529	+	330	WP_000450409.1	DUF496 family protein	-
F11EC_RS24790 (1072630)	1072630..1072953	-	324	WP_223216378.1	EutP/PduV family microcompartment system protein	-
F11EC_RS24795 (1072932)	1072932..1073012	+	81	WP_023441679.1	hypothetical protein	-
F11EC_RS04985 (1073301)	1073301..1073381	-	81	Protein_976	hypothetical protein	-
F11EC_RS04990 (1073427)	1073427..1073621	-	195	WP_000988601.1	DUF5983 family protein	-
F11EC_RS04995 (1073618)	1073618..1073992	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
F11EC_RS05000 (1074081)	1074081..1074449	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F11EC_RS05005 (1074523)	1074523..1074744	-	222	WP_000692346.1	DUF987 domain-containing protein	-
F11EC_RS05010 (1074813)	1074813..1075289	-	477	WP_178324144.1	RadC family protein	-
F11EC_RS05015 (1075305)	1075305..1075778	-	474	WP_000855074.1	antirestriction protein	-
F11EC_RS05020 (1076041)	1076041..1076862	-	822	WP_001234571.1	DUF932 domain-containing protein	-
F11EC_RS05025 (1077083)	1077083..1077493	-	411	WP_000846704.1	hypothetical protein	-
F11EC_RS05030 (1077509)	1077509..1078186	-	678	WP_001297911.1	hypothetical protein	-
F11EC_RS05035 (1078322)	1078322..1079392	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T204658 WP_000854814.1 NZ_CP076123:c1073992-1073618 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T204658 NZ_CP101197:c4587611-4587438 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT204658 WP_024174331.1 NZ_CP076123:c1074449-1074081 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT204658 NZ_CP101197:c4587935-4587639 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50