Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51932..52482 Replicon chromosome
Accession NZ_CP076123
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag F11EC_RS00240 Protein ID WP_001160966.1
Coordinates 52168..52482 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag F11EC_RS00235 Protein ID WP_000125568.1
Coordinates 51932..52165 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS00210 (47005) 47005..47292 + 288 WP_000203741.1 ferredoxin-like protein FixX -
F11EC_RS00215 (47351) 47351..48682 + 1332 WP_001183198.1 MFS transporter -
F11EC_RS00220 (48790) 48790..49320 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
F11EC_RS00225 (49313) 49313..51175 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
F11EC_RS00230 (51367) 51367..51846 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
F11EC_RS00235 (51932) 51932..52165 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
F11EC_RS00240 (52168) 52168..52482 + 315 WP_001160966.1 CcdB family protein Toxin
F11EC_RS00245 (52479) 52479..53327 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
F11EC_RS00250 (53334) 53334..53711 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
F11EC_RS00255 (53714) 53714..54535 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
F11EC_RS00260 (54532) 54532..55521 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
F11EC_RS00265 (55521) 55521..56807 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T204655 WP_001160966.1 NZ_CP076123:52168-52482 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T204655 NZ_CP101197:c4413651-4413361 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT204655 WP_000125568.1 NZ_CP076123:51932-52165 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT204655 NZ_CP101197:c4413883-4413641 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References