Detailed information of TA system TA204651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1351706..1352316
• Replicon: plasmid megaplasmid
• Accession: NZ_CP076122
• Organism: Ralstonia solanacearum strain RS24

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A454TTM1
• Locus tag: KM864_RS24265
• Protein ID: WP_058907722.1
• Coordinates: 1352008..1352316 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KM864_RS24260
• Protein ID: WP_016722339.1
• Coordinates: 1351706..1352011 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM864_RS24230 (KM864_24230)	1347150..1347689	+	540	WP_223259726.1	hypothetical protein	-
KM864_RS24235 (KM864_24235)	1348020..1348301	-	282	WP_058907724.1	hypothetical protein	-
KM864_RS24240 (KM864_24240)	1348433..1348717	-	285	WP_011003391.1	hypothetical protein	-
KM864_RS24245 (KM864_24245)	1349088..1349239	+	152	Protein_1082	IS5/IS1182 family transposase	-
KM864_RS24250 (KM864_24250)	1349379..1350074	+	696	WP_234498868.1	hypothetical protein	-
KM864_RS24255 (KM864_24255)	1350180..1351493	-	1314	WP_071507363.1	MFS family transporter	-
KM864_RS24260 (KM864_24260)	1351706..1352011	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
KM864_RS24265 (KM864_24265)	1352008..1352316	-	309	WP_058907722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KM864_RS24270 (KM864_24270)	1352392..1353591	-	1200	WP_058907721.1	MFS transporter	-
KM864_RS24275 (KM864_24275)	1353718..1354539	-	822	WP_011003398.1	GntR family transcriptional regulator	-
KM864_RS24280 (KM864_24280)	1354717..1355268	-	552	WP_011003399.1	helix-turn-helix domain-containing protein	-
KM864_RS24285 (KM864_24285)	1355401..1356000	+	600	WP_071507364.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / adeG / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI	1..2077399	2077399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11863.66 Da
• Isoelectric Point: 7.8930

>T204651 WP_058907722.1 NZ_CP076122:c1352316-1352008 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T204651 NZ_CP101197:3331422-3331808 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT204651 WP_016722339.1 NZ_CP076122:c1352011-1351706 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT204651 NZ_CP101197:3331798-3332127 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454TTM1