Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2158153..2159739 Replicon chromosome
Accession NZ_CP076120
Organism Ralstonia solanacearum strain RS24

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag KM864_RS09815 Protein ID WP_071507716.1
Coordinates 2158402..2159739 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag KM864_RS09810 Protein ID WP_028853251.1
Coordinates 2158153..2158398 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM864_RS09785 (KM864_09785) 2154216..2155142 - 927 WP_011001401.1 muramoyltetrapeptide carboxypeptidase -
KM864_RS09790 (KM864_09790) 2155139..2155690 - 552 WP_016727233.1 tRNA adenosine(34) deaminase TadA -
KM864_RS09795 (KM864_09795) 2155710..2156186 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
KM864_RS09800 (KM864_09800) 2156254..2156892 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
KM864_RS27305 2157064..2157165 + 102 Protein_1944 2-hydroxychromene-2-carboxylate isomerase -
KM864_RS09805 (KM864_09805) 2157210..2158049 + 840 WP_011001397.1 oxaloacetate decarboxylase -
KM864_RS09810 (KM864_09810) 2158153..2158398 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
KM864_RS09815 (KM864_09815) 2158402..2159739 + 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
KM864_RS09820 (KM864_09820) 2159736..2160938 + 1203 WP_071507715.1 MFS transporter -
KM864_RS09825 (KM864_09825) 2161164..2161892 + 729 WP_011001393.1 DsbA family protein -
KM864_RS09830 (KM864_09830) 2161915..2162511 - 597 WP_071507714.1 RNA 2',3'-cyclic phosphodiesterase -
KM864_RS09835 (KM864_09835) 2162659..2163357 - 699 WP_071507713.1 protocatechuate 3,4-dioxygenase subunit alpha -
KM864_RS09840 (KM864_09840) 2163354..2164094 - 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T204650 WP_071507716.1 NZ_CP076120:2158402-2159739 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T204650 NZ_CP101197:3076292-3076786 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT204650 WP_028853251.1 NZ_CP076120:2158153-2158398 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT204650 NZ_CP101197:3076002-3076295 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References