Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 437209..437842 Replicon chromosome
Accession NZ_CP076120
Organism Ralstonia solanacearum strain RS24

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag KM864_RS01990 Protein ID WP_016725172.1
Coordinates 437429..437842 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag KM864_RS01985 Protein ID WP_011002949.1
Coordinates 437209..437442 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM864_RS01970 (KM864_01970) 432797..434092 - 1296 WP_011002952.1 MFS transporter -
KM864_RS01975 (KM864_01975) 434100..435332 - 1233 WP_071507957.1 AGE family epimerase/isomerase -
KM864_RS01980 (KM864_01980) 435545..437152 + 1608 WP_019718919.1 glucan biosynthesis protein -
KM864_RS01985 (KM864_01985) 437209..437442 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KM864_RS01990 (KM864_01990) 437429..437842 + 414 WP_016725172.1 PIN domain-containing protein Toxin
KM864_RS01995 (KM864_01995) 437817..438443 - 627 WP_019718918.1 DUF1415 family protein -
KM864_RS02000 (KM864_02000) 438634..439005 + 372 WP_071507958.1 hypothetical protein -
KM864_RS02005 (KM864_02005) 439067..440563 - 1497 WP_011002945.1 glycerol kinase GlpK -
KM864_RS02010 (KM864_02010) 440725..442461 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T204649 WP_016725172.1 NZ_CP076120:437429-437842 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T204649 NZ_CP101197:2889574-2889681 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT204649 WP_011002949.1 NZ_CP076120:437209-437442 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT204649 NZ_CP101197:c2889524-2889461 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References