204648

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1318165..1319082 Replicon chromosome
Accession NZ_CP076119
Organism Bacillus velezensis strain NZ4

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag KM132_RS06920 Protein ID WP_007407256.1
Coordinates 1318336..1319082 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag KM132_RS06915 Protein ID WP_003154807.1
Coordinates 1318165..1318335 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM132_RS06880 (KM132_06880) 1313377..1314966 + 1590 WP_029973066.1 hypothetical protein -
KM132_RS06885 (KM132_06885) 1314979..1315404 + 426 WP_021493823.1 hypothetical protein -
KM132_RS06890 (KM132_06890) 1315409..1315606 + 198 WP_007610833.1 XkdX family protein -
KM132_RS06895 (KM132_06895) 1315663..1316424 + 762 WP_094295696.1 phage portal protein -
KM132_RS06900 (KM132_06900) 1316476..1316739 + 264 WP_022553473.1 hemolysin XhlA family protein -
KM132_RS06905 (KM132_06905) 1316753..1317016 + 264 WP_003154813.1 phage holin -
KM132_RS06910 (KM132_06910) 1317030..1317908 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
KM132_RS20225 1317943..1318068 - 126 WP_003154809.1 hypothetical protein -
KM132_RS06915 (KM132_06915) 1318165..1318335 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
KM132_RS06920 (KM132_06920) 1318336..1319082 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
KM132_RS06925 (KM132_06925) 1319187..1320185 - 999 WP_003154805.1 inorganic phosphate transporter -
KM132_RS06930 (KM132_06930) 1320198..1320815 - 618 WP_003154804.1 DUF47 domain-containing protein -
KM132_RS06935 (KM132_06935) 1321101..1322417 - 1317 WP_007610842.1 amino acid permease -
KM132_RS06940 (KM132_06940) 1322740..1323690 + 951 WP_007610844.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1283726..1355981 72255


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T204648 WP_007407256.1 NZ_CP076119:c1319082-1318336 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T204648 NZ_CP101197:2889039-2889146 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT204648 WP_003154807.1 NZ_CP076119:c1318335-1318165 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT204648 NZ_CP101197:c2888992-2888926 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References