Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 498951..499588 | Replicon | chromosome |
| Accession | NZ_CP076119 | ||
| Organism | Bacillus velezensis strain NZ4 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KM132_RS02520 | Protein ID | WP_003156187.1 |
| Coordinates | 499238..499588 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | KM132_RS02515 | Protein ID | WP_003156188.1 |
| Coordinates | 498951..499232 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KM132_RS02490 (KM132_02490) | 494126..495276 | + | 1151 | WP_099721935.1 | IS3 family transposase | - |
| KM132_RS02495 (KM132_02495) | 495316..495915 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| KM132_RS02500 (KM132_02500) | 496008..496373 | + | 366 | WP_014304402.1 | holo-ACP synthase | - |
| KM132_RS02505 (KM132_02505) | 496538..497545 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| KM132_RS02510 (KM132_02510) | 497662..498831 | + | 1170 | WP_215522598.1 | alanine racemase | - |
| KM132_RS02515 (KM132_02515) | 498951..499232 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KM132_RS02520 (KM132_02520) | 499238..499588 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KM132_RS02525 (KM132_02525) | 499706..500527 | + | 822 | WP_015416874.1 | STAS domain-containing protein | - |
| KM132_RS02530 (KM132_02530) | 500532..500897 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| KM132_RS02535 (KM132_02535) | 500900..501301 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| KM132_RS02540 (KM132_02540) | 501313..502320 | + | 1008 | WP_015416875.1 | PP2C family protein-serine/threonine phosphatase | - |
| KM132_RS02545 (KM132_02545) | 502384..502713 | + | 330 | WP_033575044.1 | anti-sigma factor antagonist | - |
| KM132_RS02550 (KM132_02550) | 502710..503192 | + | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| KM132_RS02555 (KM132_02555) | 503158..503946 | + | 789 | WP_029974100.1 | RNA polymerase sigma factor SigB | - |
| KM132_RS02560 (KM132_02560) | 503946..504548 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T204647 WP_003156187.1 NZ_CP076119:499238-499588 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T204647 NZ_CP101197:c2642019-2641843 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT204647 WP_003156188.1 NZ_CP076119:498951-499232 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT204647 NZ_CP101197:c2641797-2641381 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|