Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489926..490563 Replicon chromosome
Accession NZ_CP076117
Organism Bacillus amyloliquefaciens strain CQN-2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KMZ31_RS02540 Protein ID WP_003156187.1
Coordinates 490213..490563 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag KMZ31_RS02535 Protein ID WP_003156188.1
Coordinates 489926..490207 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KMZ31_RS02515 (KMZ31_02460) 486291..486890 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
KMZ31_RS02520 (KMZ31_02465) 486983..487348 + 366 WP_007609580.1 holo-ACP synthase -
KMZ31_RS02525 (KMZ31_02470) 487513..488520 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
KMZ31_RS02530 (KMZ31_02475) 488637..489806 + 1170 WP_032873001.1 alanine racemase -
KMZ31_RS02535 (KMZ31_02480) 489926..490207 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KMZ31_RS02540 (KMZ31_02485) 490213..490563 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KMZ31_RS02545 (KMZ31_02490) 490681..491502 + 822 WP_003156182.1 STAS domain-containing protein -
KMZ31_RS02550 (KMZ31_02495) 491507..491872 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
KMZ31_RS02555 (KMZ31_02500) 491875..492276 + 402 WP_003156178.1 anti-sigma regulatory factor -
KMZ31_RS02560 (KMZ31_02505) 492288..493295 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
KMZ31_RS02565 (KMZ31_02510) 493359..493688 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
KMZ31_RS02570 (KMZ31_02515) 493685..494167 + 483 WP_007609591.1 anti-sigma B factor RsbW -
KMZ31_RS02575 (KMZ31_02520) 494133..494921 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
KMZ31_RS02580 (KMZ31_02525) 494921..495523 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T204645 WP_003156187.1 NZ_CP076117:490213-490563 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T204645 NZ_CP101197:2221766-2221869 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT204645 WP_003156188.1 NZ_CP076117:489926-490207 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT204645 NZ_CP101197:c2221907-2221636 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References