Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3251740..3252373 Replicon chromosome
Accession NZ_CP076115
Organism Ralstonia solanacearum strain Rs-SY1

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag KME70_RS15335 Protein ID WP_016725172.1
Coordinates 3251740..3252153 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag KME70_RS15340 Protein ID WP_011002949.1
Coordinates 3252140..3252373 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KME70_RS15315 (KME70_15315) 3247122..3248858 + 1737 WP_058908046.1 ABC transporter substrate-binding protein -
KME70_RS15320 (KME70_15320) 3249020..3250516 + 1497 WP_011002945.1 glycerol kinase GlpK -
KME70_RS15325 (KME70_15325) 3250577..3250948 - 372 WP_011002946.1 hypothetical protein -
KME70_RS15330 (KME70_15330) 3251139..3251765 + 627 WP_058908047.1 DUF1415 domain-containing protein -
KME70_RS15335 (KME70_15335) 3251740..3252153 - 414 WP_016725172.1 PIN domain-containing protein Toxin
KME70_RS15340 (KME70_15340) 3252140..3252373 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KME70_RS15345 (KME70_15345) 3252430..3254037 - 1608 WP_058908048.1 glucan biosynthesis protein -
KME70_RS15350 (KME70_15350) 3254250..3255482 + 1233 WP_058908049.1 AGE family epimerase/isomerase -
KME70_RS15355 (KME70_15355) 3255490..3256785 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T204643 WP_016725172.1 NZ_CP076115:c3252153-3251740 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T204643 NZ_CP101197:1123528-1123863 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT204643 WP_011002949.1 NZ_CP076115:c3252373-3252140 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT204643 NZ_CP101197:1123280-1123528 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References