Detailed information of TA system TA204630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1852738..1853514
• Replicon: chromosome
• Accession: NZ_CP076105
• Organism: Staphylococcus aureus subsp. aureus RN4220

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KMZ21_RS08895
• Protein ID: WP_000031108.1
• Coordinates: 1852738..1852890 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: KMZ21_RS08900
• Protein ID: WP_001251224.1
• Coordinates: 1852915..1853514 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ21_RS08880 (KMZ21_08880)	1848697..1849518	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
KMZ21_RS08885 (KMZ21_08885)	1849981..1851366	-	1386	WP_000116224.1	class II fumarate hydratase	-
KMZ21_RS08890 (KMZ21_08890)	1851562..1851957	-	396	WP_000901021.1	hypothetical protein	-
KMZ21_RS08895 (KMZ21_08895)	1852738..1852890	-	153	WP_000031108.1	SAS053 family protein	Toxin
KMZ21_RS08900 (KMZ21_08900)	1852915..1853514	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
KMZ21_RS08905 (KMZ21_08905)	1853673..1854143	-	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KMZ21_RS08910 (KMZ21_08910)	1854148..1855275	-	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
KMZ21_RS08915 (KMZ21_08915)	1855426..1856148	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KMZ21_RS08920 (KMZ21_08920)	1856141..1857598	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T204630 WP_000031108.1 NZ_CP076105:c1852890-1852738 [Staphylococcus aureus subsp. aureus RN4220]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T204630 NZ_CP101178:c1273541-1273050 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCTTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT204630 WP_001251224.1 NZ_CP076105:c1853514-1852915 [Staphylococcus aureus subsp. aureus RN4220]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT204630 NZ_CP101178:c1273828-1273538 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4