Detailed information of TA system TA204609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 311626..312254
• Replicon: plasmid unnamed2
• Accession: NZ_CP076047
• Organism: Rhodococcus sp. USK10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I1A62_RS02100
• Protein ID: WP_219453792.1
• Coordinates: 311844..312254 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I1A62_RS02095
• Protein ID: WP_219453791.1
• Coordinates: 311626..311847 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I1A62_RS02070 (I1A62_02075)	307014..307475	+	462	WP_219455023.1	SRPBCC family protein	-
I1A62_RS45710	307559..307693	+	135	WP_257890363.1	hypothetical protein	-
I1A62_RS45715	308090..308318	-	229	Protein_316	toxin	-
I1A62_RS02075 (I1A62_02080)	308315..308533	-	219	WP_219453786.1	ribbon-helix-helix domain-containing protein	-
I1A62_RS02080 (I1A62_02085)	309318..309623	+	306	WP_219453787.1	hypothetical protein	-
I1A62_RS02085 (I1A62_02090)	309878..310327	+	450	WP_219453789.1	hypothetical protein	-
I1A62_RS45720	310468..310713	-	246	WP_257890535.1	hypothetical protein	-
I1A62_RS45725	310917..311218	-	302	Protein_321	recombinase family protein	-
I1A62_RS02095 (I1A62_02100)	311626..311847	+	222	WP_219453791.1	ribbon-helix-helix protein, CopG family	Antitoxin
I1A62_RS02100 (I1A62_02105)	311844..312254	+	411	WP_219453792.1	PIN domain nuclease	Toxin
I1A62_RS02105 (I1A62_02110)	312662..313246	-	585	WP_219453794.1	transglycosylase family protein	-
I1A62_RS02110 (I1A62_02115)	313836..314474	+	639	WP_219453796.1	pyridoxamine 5'-phosphate oxidase family protein	-
I1A62_RS02115 (I1A62_02120)	315329..315622	+	294	WP_257890364.1	WGxxGxxG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1355759	1355759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-124)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-40)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15072.28 Da
• Isoelectric Point: 6.4826

>T204609 WP_219453792.1 NZ_CP076047:311844-312254 [Rhodococcus sp. USK10]
MSDPTWLIDKSAMVRLGTTPDAEQWANRIQRGLVHICTVTLLEVGYSARTAHDLTRGRRTPPLSAMPLEYSTPAIEDRAL
EVQRQLAERGQHRAPSIPDLLIAATAEIGNRVVLHLDKDFDLIAAITGQPLERLVT

Nucleotide

• Length: 411 bp

>T204609 NZ_CP101135:2350371-2351108 [Bacillus paranthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACATCAATCATTCAAAATGTCAATCCAGTAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGTATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8405.43 Da
• Isoelectric Point: 4.3939

>AT204609 WP_219453791.1 NZ_CP076047:311626-311847 [Rhodococcus sp. USK10]
MSDVLIRNFPAEDLALLDEHAARLGISRTEFLRRQLQQEARRTITTVSTADLVRFAQDFPDLADDEVMRDAWS

Nucleotide

• Length: 222 bp

>AT204609 NZ_CP101135:2351221-2351346 [Bacillus paranthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA