Detailed information of TA system TA204608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 957973..958890
• Replicon: chromosome
• Accession: NZ_CP076045
• Organism: Bacillus velezensis strain VCN56

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KH238_RS05025
• Protein ID: WP_032866111.1
• Coordinates: 958144..958890 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KH238_RS05020
• Protein ID: WP_003154807.1
• Coordinates: 957973..958143 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH238_RS04985 (KH238_04985)	953206..954828	+	1623	WP_032866113.1	pyocin knob domain-containing protein	-
KH238_RS04990 (KH238_04990)	954841..955212	+	372	WP_020955692.1	XkdW family protein	-
KH238_RS04995 (KH238_04995)	955217..955414	+	198	WP_020955693.1	XkdX family protein	-
KH238_RS05000 (KH238_05000)	955471..956232	+	762	WP_020955694.1	hypothetical protein	-
KH238_RS05005 (KH238_05005)	956284..956547	+	264	WP_032866112.1	hemolysin XhlA family protein	-
KH238_RS05010 (KH238_05010)	956561..956824	+	264	WP_003154813.1	phage holin	-
KH238_RS05015 (KH238_05015)	956838..957716	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
KH238_RS05020 (KH238_05020)	957973..958143	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KH238_RS05025 (KH238_05025)	958144..958890	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KH238_RS05030 (KH238_05030)	958994..959992	-	999	WP_020955696.1	inorganic phosphate transporter	-
KH238_RS05035 (KH238_05035)	960005..960622	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KH238_RS05040 (KH238_05040)	960908..962224	-	1317	WP_007610842.1	amino acid permease	-
KH238_RS05045 (KH238_05045)	962547..963497	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	924497..995086	70589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T204608 WP_032866111.1 NZ_CP076045:c958890-958144 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T204608 NZ_CP101135:252689-253039 [Bacillus paranthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT204608 WP_003154807.1 NZ_CP076045:c958143-957973 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT204608 NZ_CP101135:252397-252684 [Bacillus paranthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14