Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 168265..168902 | Replicon | chromosome |
| Accession | NZ_CP076045 | ||
| Organism | Bacillus velezensis strain VCN56 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KH238_RS00760 | Protein ID | WP_003156187.1 |
| Coordinates | 168552..168902 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | KH238_RS00755 | Protein ID | WP_003156188.1 |
| Coordinates | 168265..168546 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KH238_RS00735 (KH238_00735) | 164630..165229 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| KH238_RS00740 (KH238_00740) | 165322..165687 | + | 366 | WP_007410229.1 | holo-ACP synthase | - |
| KH238_RS00745 (KH238_00745) | 165852..166859 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| KH238_RS00750 (KH238_00750) | 166976..168145 | + | 1170 | WP_032866888.1 | alanine racemase | - |
| KH238_RS00755 (KH238_00755) | 168265..168546 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KH238_RS00760 (KH238_00760) | 168552..168902 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KH238_RS00765 (KH238_00765) | 169020..169841 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| KH238_RS00770 (KH238_00770) | 169846..170211 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| KH238_RS00775 (KH238_00775) | 170214..170615 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| KH238_RS00780 (KH238_00780) | 170627..171634 | + | 1008 | WP_007410233.1 | PP2C family protein-serine/threonine phosphatase | - |
| KH238_RS00785 (KH238_00785) | 171698..172027 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| KH238_RS00790 (KH238_00790) | 172024..172506 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| KH238_RS00795 (KH238_00795) | 172472..173260 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| KH238_RS00800 (KH238_00800) | 173260..173862 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T204607 WP_003156187.1 NZ_CP076045:168552-168902 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T204607 NZ_CP101130:c1434190-1433840 [Bacillus sp. KRF7]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGATATGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATC
CGGACGATTGATAAGCAAAGATTAACGGACAAAATCACTCATCTCGATGATGAAATGATGGAAAAGGTCAATGAAGCCTT
ACAAATCAGTTTGGCACTTATCGATTTTTAA
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGATATGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATC
CGGACGATTGATAAGCAAAGATTAACGGACAAAATCACTCATCTCGATGATGAAATGATGGAAAAGGTCAATGAAGCCTT
ACAAATCAGTTTGGCACTTATCGATTTTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT204607 WP_003156188.1 NZ_CP076045:168265-168546 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT204607 NZ_CP101130:c1434476-1434195 [Bacillus sp. KRF7]
TTGTCTGAATCCAGCGCTACAACTGAAATTTTGATTCGCTTGCCAGAGGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGTGAACGCAAAAATCGACAAATTC
GAGAATCGATGAGACGCGGTTATATGGAGATGGCAAAGATCAATTTGAACATCTCTTCAGAGGCACAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
TTGTCTGAATCCAGCGCTACAACTGAAATTTTGATTCGCTTGCCAGAGGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGTGAACGCAAAAATCGACAAATTC
GAGAATCGATGAGACGCGGTTATATGGAGATGGCAAAGATCAATTTGAACATCTCTTCAGAGGCACAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|