Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4244416..4245070 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ITN70_RS20590 Protein ID WP_000244777.1
Coordinates 4244663..4245070 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ITN70_RS20585 Protein ID WP_000354046.1
Coordinates 4244416..4244682 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS20560 (4239585) 4239585..4240328 + 744 WP_000951964.1 SDR family oxidoreductase -
ITN70_RS20565 (4240385) 4240385..4241818 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ITN70_RS20570 (4241863) 4241863..4242174 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ITN70_RS20575 (4242338) 4242338..4242997 + 660 WP_000250274.1 hemolysin III family protein -
ITN70_RS20580 (4243193) 4243193..4244173 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ITN70_RS20585 (4244416) 4244416..4244682 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ITN70_RS20590 (4244663) 4244663..4245070 + 408 WP_000244777.1 protein YgfX Toxin
ITN70_RS20595 (4245110) 4245110..4245631 - 522 WP_001055874.1 flavodoxin FldB -
ITN70_RS20600 (4245743) 4245743..4246639 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ITN70_RS20605 (4246664) 4246664..4247374 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ITN70_RS20610 (4247380) 4247380..4249113 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204601 WP_000244777.1 NZ_CP076043:4244663-4245070 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204601 NZ_CP101125:4362996-4363571 [Pseudomonas nunensis]
GTGAGGCACTCCCCGTTTACCGCTGTATATGACGCATGTGTTTTGTACCCAGCCCCTTTGAGAGACTTCCTGATGTGGCT
CGCGCTCTCAGGACGTTTCCGGGCGCGATGGTCACTGGAGATACACAACGAATGGAAGCGCAATCTATTAAAGAACCGTC
CAGACCTGACCACGGAACAACTGGATCGAACCTCCGAGTTGATGGATCAAGCCATCCCCGACGCCAATGTCTCTGGATAC
GAAGACCTGATTCAAGGACTAACGCTGCCGGACATGAATGACCGCCATGTCCTGGCGGCTGCCATTCGCTGCAACGCCAG
CGTCATCGTGACCTTCAACCAGAAAGACTTCCCCTGCACATCGCTGGAACCTTTTGGAGTGGAGGCGCAGCACCCTGACA
AGTTCGTCGACAACCTCTTTGATCTGGACCCGGCAGCCGTTGTCGGTGCGGCCCAACGGCAACGCAAGCAATTGAAGATG
CCGTCGATGGATGTCAGTACCTATCTCGATCTGCTGTCACGACAAGGCTTGGTTCGGTCAGTCGAGGCGCTGACCAACTA
CCGCACCATTCTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204601 WP_000354046.1 NZ_CP076043:4244416-4244682 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204601 NZ_CP101125:4362532-4362999 [Pseudomonas nunensis]
ATGTCTTTAGTCATCCATCCACCGATCAATCTGCCCATAGCCCGCGAAGTTCAAGCTGCCATCGAAGGTCAACGTGCGCT
CTCTGCGTATCTCGCGACGCAATTCGAAACTCAACGCATCCAGATTTTCGACGAACAAAATGAAGCTCACAACGTCGAGC
TACCGACTTCAGCCCTGCGATTGCTGGTTGACATCTTGGCCGCATTGGCAGAAGGCAATGCCGTCAAAGTCGTCCCGGTT
CACGCGGAATTGACCACCCAGGAAGCAGCGGACCTGCTCAACGTCTCACGCCCGCATCTCATCAAACTGCTGGAATCCGG
CGAGTTGTCCTACCACAAAACAGGCAAGCACCGACGTGTGCGCTTTGCCGATTTGATGGATTATAAAAACCGGCGCGACA
CCGCCAGCGAGCAAGCAATGACGCTTCTCGCCGAGCAAGCACAAGCGTTAAGGACAGGATACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References