Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2854012..2854607 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag ITN70_RS13955 Protein ID WP_000239577.1
Coordinates 2854012..2854362 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ITN70_RS13960 Protein ID WP_001223208.1
Coordinates 2854356..2854607 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS13935 (2849458) 2849458..2850480 - 1023 WP_001313531.1 ABC transporter permease -
ITN70_RS13940 (2850494) 2850494..2851996 - 1503 WP_000205825.1 sugar ABC transporter ATP-binding protein -
ITN70_RS13945 (2852136) 2852136..2853092 - 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ITN70_RS13950 (2853402) 2853402..2853932 + 531 WP_000055075.1 inorganic diphosphatase -
ITN70_RS13955 (2854012) 2854012..2854362 - 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
ITN70_RS13960 (2854356) 2854356..2854607 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ITN70_RS13965 (2854819) 2854819..2855160 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ITN70_RS13970 (2855163) 2855163..2858942 - 3780 WP_000060911.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T204596 WP_000239577.1 NZ_CP076043:c2854362-2854012 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T204596 NZ_CP101124:c2353598-2353332 [Staphylococcus aureus subsp. aureus RN4220]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT204596 WP_001223208.1 NZ_CP076043:c2854607-2854356 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT204596 NZ_CP101124:c2353849-2353598 [Staphylococcus aureus subsp. aureus RN4220]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References