Detailed information of TA system TA204595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2769001..2769815
• Replicon: chromosome
• Accession: NZ_CP076043
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: ITN70_RS13530
• Protein ID: WP_001054376.1
• Coordinates: 2769001..2769258 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: ITN70_RS13535
• Protein ID: WP_001309181.1
• Coordinates: 2769270..2769815 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITN70_RS13510 (2764289)	2764289..2765395	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
ITN70_RS13515 (2765460)	2765460..2766440	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ITN70_RS21815 (2766550)	2766550..2766755	+	206	Protein_2648	HNH endonuclease	-
ITN70_RS13520 (2767023)	2767023..2768263	-	1241	Protein_2649	helicase YjhR	-
ITN70_RS21820 (2768379)	2768379..2768510	+	132	WP_001309182.1	hypothetical protein	-
ITN70_RS13530 (2769001)	2769001..2769258	+	258	WP_001054376.1	YjhX family toxin	Toxin
ITN70_RS13535 (2769270)	2769270..2769815	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
ITN70_RS13540 (2769871)	2769871..2770617	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
ITN70_RS13545 (2770786)	2770786..2771004	+	219	Protein_2654	hypothetical protein	-
ITN70_RS13550 (2771042)	2771042..2771158	+	117	Protein_2655	VOC family protein	-
ITN70_RS13555 (2771403)	2771403..2772524	+	1122	WP_000010829.1	M42 family metallopeptidase	-
ITN70_RS13560 (2772521)	2772521..2772799	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
ITN70_RS13565 (2772811)	2772811..2774124	+	1314	WP_000460842.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T204595 WP_001054376.1 NZ_CP076043:2769001-2769258 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T204595 NZ_CP101124:c2031169-2030960 [Staphylococcus aureus subsp. aureus RN4220]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT204595 WP_001309181.1 NZ_CP076043:2769270-2769815 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT204595 NZ_CP101124:c2031474-2031181 [Staphylococcus aureus subsp. aureus RN4220]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8