Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2334233..2334851 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ITN70_RS11450 Protein ID WP_001291435.1
Coordinates 2334233..2334451 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ITN70_RS11455 Protein ID WP_000344800.1
Coordinates 2334477..2334851 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS11415 (2329522) 2329522..2330094 + 573 WP_000779842.1 YbaY family lipoprotein -
ITN70_RS11420 (2330125) 2330125..2330436 - 312 WP_000409911.1 MGMT family protein -
ITN70_RS11430 (2330815) 2330815..2331168 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ITN70_RS11435 (2331210) 2331210..2332760 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ITN70_RS11440 (2332924) 2332924..2333394 - 471 WP_000136192.1 YlaC family protein -
ITN70_RS11445 (2333510) 2333510..2334061 - 552 WP_000102564.1 maltose O-acetyltransferase -
ITN70_RS11450 (2334233) 2334233..2334451 - 219 WP_001291435.1 HHA domain-containing protein Toxin
ITN70_RS11455 (2334477) 2334477..2334851 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ITN70_RS11460 (2335397) 2335397..2338546 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
ITN70_RS11465 (2338569) 2338569..2339762 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204593 WP_001291435.1 NZ_CP076043:c2334451-2334233 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204593 NZ_CP101124:c1853408-1853256 [Staphylococcus aureus subsp. aureus RN4220]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204593 WP_000344800.1 NZ_CP076043:c2334851-2334477 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204593 NZ_CP101124:c1854032-1853433 [Staphylococcus aureus subsp. aureus RN4220]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References