Detailed information of TA system TA204592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1312786..1313424
• Replicon: chromosome
• Accession: NZ_CP076043
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ITN70_RS06485
• Protein ID: WP_000813794.1
• Coordinates: 1313248..1313424 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ITN70_RS06480
• Protein ID: WP_001270286.1
• Coordinates: 1312786..1313202 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITN70_RS06460 (1307938)	1307938..1308879	-	942	WP_001251304.1	ABC transporter permease	-
ITN70_RS06465 (1308880)	1308880..1309893	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ITN70_RS06470 (1309911)	1309911..1311056	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ITN70_RS06475 (1311301)	1311301..1312707	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ITN70_RS06480 (1312786)	1312786..1313202	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ITN70_RS06485 (1313248)	1313248..1313424	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ITN70_RS06490 (1313646)	1313646..1313876	+	231	WP_000494244.1	YncJ family protein	-
ITN70_RS06495 (1313968)	1313968..1315929	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ITN70_RS06500 (1316002)	1316002..1316538	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ITN70_RS06505 (1316630)	1316630..1317805	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204592 WP_000813794.1 NZ_CP076043:c1313424-1313248 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204592 NZ_CP101124:c812007-811819 [Staphylococcus aureus subsp. aureus RN4220]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204592 WP_001270286.1 NZ_CP076043:c1313202-1312786 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204592 NZ_CP101124:c812296-812036 [Staphylococcus aureus subsp. aureus RN4220]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 6HPB