Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 107057..107724 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag ITN70_RS00555 Protein ID WP_001094400.1
Coordinates 107057..107386 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag ITN70_RS00560 Protein ID WP_000072690.1
Coordinates 107407..107724 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS00550 (102113) 102113..106693 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
ITN70_RS00555 (107057) 107057..107386 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
ITN70_RS00560 (107407) 107407..107724 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ITN70_RS00565 (107762) 107762..107962 - 201 WP_001395452.1 DUF987 domain-containing protein -
ITN70_RS00570 (107971) 107971..108453 - 483 WP_001407480.1 RadC family protein -
ITN70_RS00575 (108462) 108462..108920 - 459 WP_000211841.1 antirestriction protein -
ITN70_RS00580 (109023) 109023..109207 - 185 Protein_109 DUF905 family protein -
ITN70_RS00585 (109818) 109818..111521 - 1704 WP_000896263.1 protein YfjW -
ITN70_RS00590 (111663) 111663..112672 + 1010 Protein_111 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T204578 WP_001094400.1 NZ_CP076043:c107386-107057 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T204578 NZ_CP101116:c2021-1734 [Acinetobacter sp. Z1]
ATGGAACTGTATTTCGAATGGGATGAGGCAAAGAACCAAAAGAATCAGAAAAAACATGATGTCTCTTTTGAAACGGCAAG
CCTCATTTTTGAAGATCCATTACGGATATCAATCCAAGATAGACATACTGATGGCGAAGAACGTTGGCAATCCATTGGTA
TGGTTAAGGGCGTACTAATGCTTTTAGTTGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTAACCAAAGCAGAGCGGGATAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT204578 WP_000072690.1 NZ_CP076043:c107724-107407 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT204578 NZ_CP101116:c1747-1436 [Acinetobacter sp. Z1]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGATAATTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCGAAAGGAACTACTCCTGATGAGCAATTAGACTTATCGGATATTCCTGAAATTACTGACTGGAGTCATGCTGTTCGTC
ATGGGCAATTCTATCGCCCAGTGAAGCAACAGACTTCTGTTCGCCTTGATGCTGATGTACTTGCATGGTTAAAAGAACAA
GGTAAAGGCTATCAGACACGCATAAATAAAATTCTGCGTGAAGCTATGTTAAAAGATTTAAAAAATCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References