Detailed information of TA system TA204572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1575245..1575774
• Replicon: chromosome
• Accession: NZ_CP076029
• Organism: Staphylococcus sp. MZ9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KI244_RS07845
• Protein ID: WP_000621175.1
• Coordinates: 1575245..1575607 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KI244_RS07850
• Protein ID: WP_000948331.1
• Coordinates: 1575604..1575774 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI244_RS07825 (KI244_07825)	1572223..1572993	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KI244_RS07830 (KI244_07830)	1572968..1573447	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KI244_RS07835 (KI244_07835)	1573449..1573775	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KI244_RS07840 (KI244_07840)	1573894..1574895	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KI244_RS07845 (KI244_07845)	1575245..1575607	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI244_RS07850 (KI244_07850)	1575604..1575774	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KI244_RS07855 (KI244_07855)	1575859..1577007	-	1149	WP_001281154.1	alanine racemase	-
KI244_RS07860 (KI244_07860)	1577073..1577432	-	360	WP_000581197.1	holo-ACP synthase	-
KI244_RS07865 (KI244_07865)	1577436..1577927	-	492	WP_072353916.1	PH domain-containing protein	-
KI244_RS07870 (KI244_07870)	1577914..1579497	-	1584	WP_045177766.1	PH domain-containing protein	-
KI244_RS07875 (KI244_07875)	1579490..1579969	-	480	WP_001287079.1	hypothetical protein	-
KI244_RS07880 (KI244_07880)	1580178..1580738	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T204572 WP_000621175.1 NZ_CP076029:c1575607-1575245 [Staphylococcus sp. MZ9]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T204572 NZ_CP101114:c597327-597001 [Bartonella harrusi]
ATGAAGCGTGGATCTCTCGTAACAATAGCGATGCAGGGTGATTTTGGTAAACCAAGACCTGCATTAATAATTCAAGCCAA
TCAATTCAGTGAACATACAAGCGTAACAGTTTTACCAATTACAAGTACACTTATTGCAGCGCCATTACTTCGCATTACTA
TTCAACCAGATGCCAAAAATGGTTTACAAAAGCTTTCACAAGTGATGATTGATAAGATCATGACGGTAAGATGCGAAAAG
GTTAGCCCAGCTTTCGGTTCTATTCATGCAGATAAAATGGTAGAAATTGAACGCTGTTTGGCTGTGTTTTTGGGAATAGT
AAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT204572 WP_000948331.1 NZ_CP076029:c1575774-1575604 [Staphylococcus sp. MZ9]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT204572 NZ_CP101114:c597548-597324 [Bartonella harrusi]
ATGGCTACAATGCACGTCAATGAACGCGTTCAAAAACATCGTAACGCACAAAGGAAAGCAGGATTGCGCTTGATGCAAAT
TTGGGTACCAGATACACGCCAACCAAACTTTGCAGAAGAGTGCCGCCGGCAGTGTCGCTTGGTAGCAAAAATGGATAAAA
CAGATACATCTATGCAATTATTTATGGATCAATCTTTAGTGGAAGTTGATGGCTGGACAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1