Detailed information of TA system TA204561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2143610..2144139
• Replicon: chromosome
• Accession: NZ_CP076028
• Organism: Staphylococcus sp. MZ8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KI239_RS10515
• Protein ID: WP_000621175.1
• Coordinates: 2143610..2143972 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KI239_RS10520
• Protein ID: WP_000948331.1
• Coordinates: 2143969..2144139 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI239_RS10495 (KI239_10495)	2140588..2141358	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KI239_RS10500 (KI239_10500)	2141333..2141812	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KI239_RS10505 (KI239_10505)	2141814..2142140	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KI239_RS10510 (KI239_10510)	2142259..2143260	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KI239_RS10515 (KI239_10515)	2143610..2143972	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI239_RS10520 (KI239_10520)	2143969..2144139	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KI239_RS10525 (KI239_10525)	2144224..2145372	-	1149	WP_001281154.1	alanine racemase	-
KI239_RS10530 (KI239_10530)	2145438..2145797	-	360	WP_000581197.1	holo-ACP synthase	-
KI239_RS10535 (KI239_10535)	2145801..2146292	-	492	WP_072353916.1	PH domain-containing protein	-
KI239_RS10540 (KI239_10540)	2146279..2147862	-	1584	WP_045177766.1	PH domain-containing protein	-
KI239_RS10545 (KI239_10545)	2147855..2148334	-	480	WP_001287079.1	hypothetical protein	-
KI239_RS10550 (KI239_10550)	2148543..2149103	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T204561 WP_000621175.1 NZ_CP076028:c2143972-2143610 [Staphylococcus sp. MZ8]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T204561 NZ_CP100765:c1751756-1751472 [Serratia nematodiphila]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCTGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT204561 WP_000948331.1 NZ_CP076028:c2144139-2143969 [Staphylococcus sp. MZ8]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT204561 NZ_CP100765:c1751997-1751746 [Serratia nematodiphila]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCAGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAGTACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1