Detailed information of TA system TA204550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2144896..2145425
• Replicon: chromosome
• Accession: NZ_CP076027
• Organism: Staphylococcus sp. MZ7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KI234_RS10585
• Protein ID: WP_000621175.1
• Coordinates: 2144896..2145258 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KI234_RS10590
• Protein ID: WP_000948331.1
• Coordinates: 2145255..2145425 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI234_RS10565 (KI234_10565)	2141874..2142644	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KI234_RS10570 (KI234_10570)	2142619..2143098	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KI234_RS10575 (KI234_10575)	2143100..2143426	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KI234_RS10580 (KI234_10580)	2143545..2144546	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KI234_RS10585 (KI234_10585)	2144896..2145258	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI234_RS10590 (KI234_10590)	2145255..2145425	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KI234_RS10595 (KI234_10595)	2145510..2146658	-	1149	WP_001281154.1	alanine racemase	-
KI234_RS10600 (KI234_10600)	2146724..2147083	-	360	WP_000581197.1	holo-ACP synthase	-
KI234_RS10605 (KI234_10605)	2147087..2147578	-	492	WP_072353916.1	PH domain-containing protein	-
KI234_RS10610 (KI234_10610)	2147565..2149148	-	1584	WP_045177766.1	PH domain-containing protein	-
KI234_RS10615 (KI234_10615)	2149141..2149620	-	480	WP_001287079.1	hypothetical protein	-
KI234_RS10620 (KI234_10620)	2149829..2150389	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T204550 WP_000621175.1 NZ_CP076027:c2145258-2144896 [Staphylococcus sp. MZ7]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T204550 NZ_CP100764:c1152168-1151965 [Serratia plymuthica]
ATGACTAAAATTGACTATCTGATGCGTTTACGTAAATGCACCACGATTGACACCCTGGAACGCGTTATTGAAAAAAATAA
GTATGAGCTTTCGGATGACGAACTAGAGCTGTTCTACTCTGCTGCCGACCACCGTTTGGCCGAGCTCACCATGAATAAGC
TGTACGATAAGATCCCTGTTGCCGTTTGGAAATTCGTACGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT204550 WP_000948331.1 NZ_CP076027:c2145425-2145255 [Staphylococcus sp. MZ7]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT204550 NZ_CP100764:c1152587-1152219 [Serratia plymuthica]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
CACGCTGGGCGACAGCCACCACGGCTGGGTGAACGATCCAACGTCTTCCGTCAACCTCCAGCTTAACGAGTTGATTGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAAAGTGATTTATCGGAGTTAGTCGAAGAGTATCTC
GACGATACCTACACGCTGTTTAGCAGTTATGGTATTAATGATTCCGATTTGCGCCGCTGGCAAAAAACCAAAGCGCGATT
ATTCAGAATGTTCTCAGGAGAGGGCATCTGTACGACAATGAAAACTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1