Detailed information of TA system TA204544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 842145..842622
• Replicon: chromosome
• Accession: NZ_CP076027
• Organism: Staphylococcus sp. MZ7

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KI234_RS03955
• Protein ID: WP_000052076.1
• Coordinates: 842145..842333 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KI234_RS03960
• Protein ID: WP_001059690.1
• Coordinates: 842362..842622 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI234_RS03925 (KI234_03925)	838187..838657	+	471	WP_000824472.1	hypothetical protein	-
KI234_RS03930 (KI234_03930)	838998..839683	+	686	Protein_772	thermonuclease family protein	-
KI234_RS03935 (KI234_03935)	840040..840240	+	201	WP_001059082.1	cold-shock protein	-
KI234_RS03940 (KI234_03940)	840736..840954	-	219	WP_000005886.1	hypothetical protein	-
KI234_RS03945 (KI234_03945)	841016..841300	-	285	WP_001004401.1	hypothetical protein	-
KI234_RS03950 (KI234_03950)	841388..841957	-	570	WP_045178121.1	lipoprotein N-acylation protein LnsA	-
KI234_RS03955 (KI234_03955)	842145..842333	-	189	WP_000052076.1	hypothetical protein	Toxin
KI234_RS03960 (KI234_03960)	842362..842622	-	261	WP_001059690.1	hypothetical protein	Antitoxin
KI234_RS03965 (KI234_03965)	842963..843166	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KI234_RS03970 (KI234_03970)	843163..843399	-	237	WP_000368687.1	hypothetical protein	-
KI234_RS03975 (KI234_03975)	843624..844205	+	582	WP_001140697.1	histidine phosphatase family protein	-
KI234_RS03980 (KI234_03980)	844278..844895	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
KI234_RS03985 (KI234_03985)	845050..845544	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
KI234_RS03990 (KI234_03990)	845943..846365	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
KI234_RS03995 (KI234_03995)	846513..847229	+	717	WP_000150017.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Antitoxin MazE-like (6-70)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T204544 WP_000052076.1 NZ_CP076027:c842333-842145 [Staphylococcus sp. MZ7]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T204544 NZ_CP100755:3844106-3844381 [Alcaligenes sp. NLF5-7]
ATGAAATTAAAGTGGACTAGTAAGGCGCTTTCTGATTTAGAGCGCCTGTATGAGTTTTTGGCCGTGGTGAATAAACCTGC
TGCTGCTCGTGCCGTGCAAAAACTGACCAAGGCCCCGAGCATTTTACTGAGCAATCCACGCATTGGTGAGCAGCTCTTTA
TGTTTGAGCCACGTGAGGTAAGGCGAATTCTGGTCGGAGAGTATGAAATTCGTTATGAGCTTCAGGATTCGTTTATCTAC
GTGCTTCGGATATGGCACACCAGAGAAAATCGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT204544 WP_001059690.1 NZ_CP076027:c842622-842362 [Staphylococcus sp. MZ7]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT204544 NZ_CP100755:3844485-3844703 [Alcaligenes sp. NLF5-7]
ATGGCAAACGTACACGCAAGAGTTCAGAAGAAGTGTGTTTTGCTCCGGCGGGCTGGGTTGCGCCCAGTGCAACTCTGGGT
GCCTGACACACGTCAGCCTAGCTTTGCGAAGGTATGCTTTCTTCAGGCGCAAATAGCAGCCAAGGCAGATAAGGTTGATG
TGGGTACAGAACATCTAATGGATAAGGCTCTAGCTGATCTGAATAGCTGGACAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56