Detailed information of TA system TA204538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2121270..2121799
• Replicon: chromosome
• Accession: NZ_CP076026
• Organism: Staphylococcus sp. MZ3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KI230_RS10375
• Protein ID: WP_000621175.1
• Coordinates: 2121270..2121632 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KI230_RS10380
• Protein ID: WP_000948331.1
• Coordinates: 2121629..2121799 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI230_RS10355 (KI230_10355)	2118248..2119018	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KI230_RS10360 (KI230_10360)	2118993..2119472	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KI230_RS10365 (KI230_10365)	2119474..2119800	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KI230_RS10370 (KI230_10370)	2119919..2120920	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KI230_RS10375 (KI230_10375)	2121270..2121632	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI230_RS10380 (KI230_10380)	2121629..2121799	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KI230_RS10385 (KI230_10385)	2121884..2123032	-	1149	WP_001281154.1	alanine racemase	-
KI230_RS10390 (KI230_10390)	2123098..2123457	-	360	WP_000581197.1	holo-ACP synthase	-
KI230_RS10395 (KI230_10395)	2123461..2123952	-	492	WP_072353916.1	PH domain-containing protein	-
KI230_RS10400 (KI230_10400)	2123939..2125522	-	1584	WP_045177766.1	PH domain-containing protein	-
KI230_RS10405 (KI230_10405)	2125515..2125994	-	480	WP_001287079.1	hypothetical protein	-
KI230_RS10410 (KI230_10410)	2126203..2126763	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T204538 WP_000621175.1 NZ_CP076026:c2121632-2121270 [Staphylococcus sp. MZ3]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T204538 NZ_CP100753:c5096391-5095912 [Serratia marcescens]
ATGATTGTGCTGGATACCAACGTCGTTTCGGAATTGCTGCGGCCGGCGCCTCACTACAATGTATTGCGCTGGTTAGATGA
GCAGGATACGTATCGGTTCTATTTGAGCGCCATCGTGGTTGCGGAGTTGTATACGGGCATCGCCTGCATGCCTCACGGCA
AGAAACAGCTCGAACTGCAAACTAACCTCGGCGACATGCTGCAGGAAGAGTTTTCAGACAGAATCTTACCCTTTGACGTC
GGCTGTGCGATGCAGCATGCGGAATTAATGGGCGCCAATCGCCGCCGAGGCATTGGCGTCAGCATGCCCGATACCCAAAT
CGCCGCCACCTGCCTGCATTATGGCGCCACCCTGGCCACCCGCAATACCAAAGACTTCCTCCACTGCGGCATCGAGTTAA
TCGATCCGTGGCAGGCGCCGACCGGCCGTCGGTTGCATGAAGATGCGGCGGAATACTACGTGATGAGCAGAAAGTCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT204538 WP_000948331.1 NZ_CP076026:c2121799-2121629 [Staphylococcus sp. MZ3]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT204538 NZ_CP100753:c5096648-5096388 [Serratia marcescens]
ATGGCGACTATCACCGTCAGAAATCTGGACGACGAAATCAAAGAACTGCTGCGTATTTCAGCGGCGAAAAACGGCCACTC
CATGGAAGAGGAAGCGCGCATGATCCTGAAGCAGGCGCTGGTGAAAAAGCCGCCGCGCTATGGGTTAGGAACACGCATGC
ATCAGTACTTCGCCGAATTCGGCGGCGTTGAGTTGGAGATCCCCCCACGTGATGAGGCCCCTCATCGTATCGTCAACTTT
GATGAAGATGATGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1