Detailed information of TA system TA204527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2094443..2094972
• Replicon: chromosome
• Accession: NZ_CP076025
• Organism: Staphylococcus sp. MZ1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KI229_RS10255
• Protein ID: WP_000621175.1
• Coordinates: 2094443..2094805 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KI229_RS10260
• Protein ID: WP_000948331.1
• Coordinates: 2094802..2094972 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI229_RS10235 (KI229_10235)	2091421..2092191	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KI229_RS10240 (KI229_10240)	2092166..2092645	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KI229_RS10245 (KI229_10245)	2092647..2092973	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KI229_RS10250 (KI229_10250)	2093092..2094093	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KI229_RS10255 (KI229_10255)	2094443..2094805	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI229_RS10260 (KI229_10260)	2094802..2094972	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KI229_RS10265 (KI229_10265)	2095057..2096205	-	1149	WP_001281154.1	alanine racemase	-
KI229_RS10270 (KI229_10270)	2096271..2096630	-	360	WP_000581197.1	holo-ACP synthase	-
KI229_RS10275 (KI229_10275)	2096634..2097125	-	492	WP_072353916.1	PH domain-containing protein	-
KI229_RS10280 (KI229_10280)	2097112..2098695	-	1584	WP_045177766.1	PH domain-containing protein	-
KI229_RS10285 (KI229_10285)	2098688..2099167	-	480	WP_001287079.1	hypothetical protein	-
KI229_RS10290 (KI229_10290)	2099376..2099936	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T204527 WP_000621175.1 NZ_CP076025:c2094805-2094443 [Staphylococcus sp. MZ1]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T204527 NZ_CP100753:c158103-157792 [Serratia marcescens]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAACATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT204527 WP_000948331.1 NZ_CP076025:c2094972-2094802 [Staphylococcus sp. MZ1]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT204527 NZ_CP100753:c157795-157373 [Serratia marcescens]
ATGATTACAGATGCTATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1