Detailed information of TA system TA204521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3076423..3077219
• Replicon: chromosome
• Accession: NZ_CP076024
• Organism: Methylobacter sp. S3L5C

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KKZ03_RS13740
• Protein ID: WP_243221628.1
• Coordinates: 3076423..3076935 (-)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: KKZ03_RS13745
• Protein ID: WP_243217391.1
• Coordinates: 3076932..3077219 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ03_RS13715 (KKZ03_13655)	3071916..3072560	-	645	WP_243217386.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
KKZ03_RS13720 (KKZ03_13660)	3072563..3073492	-	930	WP_243217387.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
KKZ03_RS13725 (KKZ03_13665)	3073571..3074710	-	1140	WP_243217388.1	beta-propeller fold lactonase family protein	-
KKZ03_RS13730 (KKZ03_13670)	3074870..3075751	+	882	WP_243217389.1	LysR substrate-binding domain-containing protein	-
KKZ03_RS13735 (KKZ03_13675)	3076012..3076233	-	222	WP_243217390.1	DUF202 domain-containing protein	-
KKZ03_RS13740 (KKZ03_13680)	3076423..3076935	-	513	WP_243221628.1	GNAT family N-acetyltransferase	Toxin
KKZ03_RS13745 (KKZ03_13685)	3076932..3077219	-	288	WP_243217391.1	DUF1778 domain-containing protein	Antitoxin
KKZ03_RS13750	3077520..3077760	+	241	Protein_2708	IS110 family transposase	-
KKZ03_RS13755 (KKZ03_13690)	3077906..3079597	-	1692	WP_243217392.1	AAA family ATPase	-
KKZ03_RS13760 (KKZ03_13695)	3079774..3081299	+	1526	Protein_2710	IS3 family transposase	-
KKZ03_RS13765 (KKZ03_13700)	3081326..3082075	-	750	WP_243217393.1	helix-hairpin-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2973874..3185041	211167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 18493.49 Da
• Isoelectric Point: 9.1131

>T204521 WP_243221628.1 NZ_CP076024:c3076935-3076423 [Methylobacter sp. S3L5C]
MIALNLTPPAPITADQKLANFDSGELSLNEWLKKRAFKNHATGASRCFVLCTGSDVIGFYSLSAGAISHEAAPKAMRRNM
PNPLPVLLLGRLAVDKRYHNQGIGQALLRDAMMRAINVSSDAGVFALLVHALSDQAKQFYLSRGFVESPLQPMTLVMTIE
TVRSILAEPD

Nucleotide

• Length: 513 bp

>T204521 NZ_CP100747:c4156997-4156506 [Salmonella enterica subsp. enterica serovar Montevideo]
ATGATTTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCAC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10928.51 Da
• Isoelectric Point: 8.0531

>AT204521 WP_243217391.1 NZ_CP076024:c3077219-3076932 [Methylobacter sp. S3L5C]
MFHAQTRETVSVSINIRAKARQRDLIDQAAERLGRSRSDFMLEAACREAEDVLLDQAFFTVDEGTFAKFHALLDQPLPPT
DKLRRLLKTKAPWEK

Nucleotide

• Length: 288 bp

>AT204521 NZ_CP100747:c4157287-4156994 [Salmonella enterica subsp. enterica serovar Montevideo]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCCTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCGGACCCCTGCGCCGTGGGAGCAGGAAAAATGA