Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32666..33309 Replicon plasmid p4WGR351
Accession NZ_CP076012
Organism Klebsiella pneumoniae subsp. pneumoniae strain WGR351

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag J4H97_RS28110 Protein ID WP_040155119.1
Coordinates 32893..33309 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag J4H97_RS28105 Protein ID WP_001261276.1
Coordinates 32666..32896 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H97_RS28070 (J4H97_28070) 27780..28049 + 270 WP_000339857.1 hypothetical protein -
J4H97_RS28075 (J4H97_28075) 28226..29092 - 867 WP_004118283.1 replication initiation protein -
J4H97_RS28080 (J4H97_28080) 29627..29731 + 105 WP_032409716.1 hypothetical protein -
J4H97_RS28085 (J4H97_28085) 29860..30117 + 258 WP_000764642.1 hypothetical protein -
J4H97_RS28090 (J4H97_28090) 30175..30951 - 777 WP_000015958.1 site-specific integrase -
J4H97_RS28095 (J4H97_28095) 30948..31691 - 744 WP_000129823.1 hypothetical protein -
J4H97_RS28100 (J4H97_28100) 31742..32092 - 351 WP_000493378.1 hypothetical protein -
J4H97_RS28105 (J4H97_28105) 32666..32896 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J4H97_RS28110 (J4H97_28110) 32893..33309 + 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
J4H97_RS28115 (J4H97_28115) 33350..34228 - 879 WP_009309916.1 restriction endonuclease -
J4H97_RS28120 (J4H97_28120) 34890..36167 + 1278 Protein_37 HlyD family secretion protein -
J4H97_RS28125 (J4H97_28125) 36157..37110 + 954 Protein_38 cysteine peptidase family C39 domain-containing protein -
J4H97_RS28130 (J4H97_28130) 37167..37864 + 698 WP_095033700.1 IS1-like element IS1B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / aph(6)-Id / aph(3'')-Ib / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 1..49065 49065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T204517 WP_040155119.1 NZ_CP076012:32893-33309 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T204517 NZ_CP100747:2032320-2032423 [Salmonella enterica subsp. enterica serovar Montevideo]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT204517 WP_001261276.1 NZ_CP076012:32666-32896 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT204517 NZ_CP100747:c2032425-2032282 [Salmonella enterica subsp. enterica serovar Montevideo]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References