Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5329056..5329681 Replicon chromosome
Accession NZ_CP076008
Organism Klebsiella pneumoniae subsp. pneumoniae strain WGR351

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J4H97_RS25835 Protein ID WP_002882817.1
Coordinates 5329056..5329439 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J4H97_RS25840 Protein ID WP_004150355.1
Coordinates 5329439..5329681 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H97_RS25820 (5326422) 5326422..5327324 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J4H97_RS25825 (5327321) 5327321..5327956 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J4H97_RS25830 (5327953) 5327953..5328882 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J4H97_RS25835 (5329056) 5329056..5329439 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J4H97_RS25840 (5329439) 5329439..5329681 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J4H97_RS25845 (5329886) 5329886..5330803 + 918 WP_021466676.1 alpha/beta hydrolase -
J4H97_RS25850 (5330817) 5330817..5331758 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J4H97_RS25855 (5331803) 5331803..5332240 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J4H97_RS25860 (5332237) 5332237..5333097 - 861 WP_004146232.1 virulence factor BrkB family protein -
J4H97_RS25865 (5333091) 5333091..5333690 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T204515 WP_002882817.1 NZ_CP076008:c5329439-5329056 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T204515 NZ_CP100747:849722-850135 [Salmonella enterica subsp. enterica serovar Montevideo]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCATCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGGGCCAACACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT204515 WP_004150355.1 NZ_CP076008:c5329681-5329439 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT204515 NZ_CP100747:849475-849741 [Salmonella enterica subsp. enterica serovar Montevideo]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References