Detailed information of TA system TA204501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1476911..1477647
• Replicon: chromosome
• Accession: NZ_CP076008
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WGR351

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: J4H97_RS07310
• Protein ID: WP_071836879.1
• Coordinates: 1477165..1477647 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: J4H97_RS07305
• Protein ID: WP_003026799.1
• Coordinates: 1476911..1477177 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4H97_RS07280 (1472557)	1472557..1473696	+	1140	WP_032433368.1	mannitol dehydrogenase	-
J4H97_RS07285 (1473725)	1473725..1474387	+	663	WP_032433366.1	dihydroxyacetone kinase subunit DhaL	-
J4H97_RS07290 (1474371)	1474371..1475375	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
J4H97_RS07295 (1475393)	1475393..1476025	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
J4H97_RS07300 (1476035)	1476035..1476598	+	564	WP_215116248.1	6-phospho-3-hexuloisomerase	-
J4H97_RS07305 (1476911)	1476911..1477177	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
J4H97_RS07310 (1477165)	1477165..1477647	+	483	WP_071836879.1	GNAT family N-acetyltransferase	Toxin
J4H97_RS07315 (1477805)	1477805..1479337	-	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
J4H97_RS07320 (1479449)	1479449..1480852	+	1404	WP_039815096.1	ISNCY-like element ISKpn21 family transposase	-
J4H97_RS07325 (1480881)	1480881..1481513	-	633	WP_001567369.1	hypothetical protein	-
J4H97_RS07330 (1481893)	1481893..1482492	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1444856..1492475	47619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17481.06 Da
• Isoelectric Point: 9.8177

>T204501 WP_071836879.1 NZ_CP076008:1477165-1477647 [Klebsiella pneumoniae subsp. pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVYKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T204501 NZ_CP100744:311331-311699 [Salmonella enterica subsp. enterica serovar Newport]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT204501 WP_003026799.1 NZ_CP076008:1476911-1477177 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT204501 NZ_CP100744:311113-311334 [Salmonella enterica subsp. enterica serovar Newport]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66