Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1460220..1460863 Replicon chromosome
Accession NZ_CP076008
Organism Klebsiella pneumoniae subsp. pneumoniae strain WGR351

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag J4H97_RS07215 Protein ID WP_032433387.1
Coordinates 1460447..1460863 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag J4H97_RS07210 Protein ID WP_001261276.1
Coordinates 1460220..1460450 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H97_RS07195 (1455242) 1455242..1456329 + 1088 Protein_1405 transcriptional regulator -
J4H97_RS07200 (1456332) 1456332..1458572 + 2241 WP_045326788.1 P-loop NTPase fold protein -
J4H97_RS07205 (1459100) 1459100..1459915 - 816 WP_032433388.1 hypothetical protein -
J4H97_RS07210 (1460220) 1460220..1460450 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J4H97_RS07215 (1460447) 1460447..1460863 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
J4H97_RS07220 (1461019) 1461019..1461999 + 981 WP_032433385.1 hypothetical protein -
J4H97_RS07225 (1462194) 1462194..1463765 - 1572 WP_032433383.1 AAA family ATPase -
J4H97_RS07230 (1464084) 1464084..1464332 + 249 WP_032433382.1 hypothetical protein -
J4H97_RS07235 (1464391) 1464391..1464909 + 519 WP_045326794.1 hypothetical protein -
J4H97_RS07240 (1464940) 1464940..1465431 + 492 WP_032433378.1 hypothetical protein -
J4H97_RS07245 (1465491) 1465491..1465694 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1444856..1492475 47619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T204500 WP_032433387.1 NZ_CP076008:1460447-1460863 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T204500 NZ_CP100741:2221-2496 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT204500 WP_001261276.1 NZ_CP076008:1460220-1460450 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT204500 NZ_CP100741:1937-2221 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAAATGAAGAACAATAGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCTCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References