Detailed information of TA system TA204495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 24856..25346
• Replicon: plasmid p2
• Accession: NZ_CP075899
• Organism: Exiguobacterium acetylicum strain SI17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KKI46_RS17165
• Protein ID: WP_214814065.1
• Coordinates: 24856..25122 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KKI46_RS17170
• Protein ID: WP_214813982.1
• Coordinates: 25122..25346 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKI46_RS17155 (KKI46_17155)	20894..22363	-	1470	WP_214814060.1	class I SAM-dependent DNA methyltransferase	-
KKI46_RS17160 (KKI46_17160)	22376..24730	-	2355	WP_214813980.1	DEAD/DEAH box helicase family protein	-
KKI46_RS17165 (KKI46_17165)	24856..25122	-	267	WP_214814065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KKI46_RS17170 (KKI46_17170)	25122..25346	-	225	WP_214813982.1	DUF6290 family protein	Antitoxin
KKI46_RS17175 (KKI46_17175)	25578..25913	-	336	WP_214813984.1	YolD-like family protein	-
KKI46_RS17180 (KKI46_17180)	26092..27084	-	993	WP_214813986.1	DNA/RNA non-specific endonuclease	-
KKI46_RS17185 (KKI46_17185)	27518..27964	+	447	WP_244989040.1	JAB domain-containing protein	-
KKI46_RS17190 (KKI46_17190)	28491..28727	-	237	WP_214813988.1	hypothetical protein	-
KKI46_RS17195 (KKI46_17195)	28924..29379	-	456	WP_214813989.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..43790	43790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10372.03 Da
• Isoelectric Point: 10.3966

>T204495 WP_214814065.1 NZ_CP075899:c25122-24856 [Exiguobacterium acetylicum]
MAYTVEFERGAQKSLKKMDPQQARIIMSWIKKNLVGTDDPRRHGKGLVSNRSGEWRYRIGDYRLIADIQDEKVVILILEI
GHRRDIYK

Nucleotide

• Length: 267 bp

>T204495 NZ_CP100739:c4143640-4143356 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8613.79 Da
• Isoelectric Point: 4.5127

>AT204495 WP_214813982.1 NZ_CP075899:c25346-25122 [Exiguobacterium acetylicum]
MSTISVRLDDQDTRLIKEYAKAKNITISTLVRDAVLDRIEDEIDLQLYHDSMAAHRKKSEAISFEDMMKELDLE

Nucleotide

• Length: 225 bp

>AT204495 NZ_CP100739:c4143872-4143630 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA